Abstract: The demarcation of ecological space and the identification and control of leading service functions are the basic premise and primary task of urban ecological space planning. Taking the Eastern New City of Chengdu as an example, this research takes the ecosystem service value as the standard to measure the ecological space stock, delimits the spatial distribution range of core ecological functions through the hot spot analysis index (Gi* index), and at the same time, the spatial locations corresponding to the dominant ecosystem service functions are screened to identify different types of ecological function spaces. Based on this, the bivariate spatial Local autocorrelation index (Local Moran’s I) was used to interpret the trade-off and collaboration mechanism among ecosystem services, and to try to put forward a new way to identify and control ecological function space from the perspective of ecological trade-off and collaboration, so as to provide a theoretical basis for the construction of new urban land space planning and ecological civilization construction in the new era.